Transformation with Stellar competent cells

Transformation with Stellar competent cells

1.    Thaw cells in ice bath

2.    Mix gently, move 50 μl into a falcon tube

3.    Add no more than 5ng DNA

4.    Let sit on ice for 30 min

5.    Heat shock at 42° for 45 seconds

6.    Ice for 1-2 min

7.    Add SOC medium (warmed to 37 degrees) to make final volume 500 μl

8.    Incubate by shaking for 1 hour at 37 degrees.

9.    Feel good about yourself for at least one hour. You’ve done a great job.