DNA Miniprep Protocol

This protocol gave us some highly concentrated samples, so feel free to try it out.
 * 1) Pour bacteria cultures into microcentrifuge tubes and centrifuge for 1 minute at 13,000 rpm.
 * 2) Discard the supernatant and refill if there is remaining liquid culture. Centrifuge again.
 * 3) Discard the supernatant. Flip the tubes upside-down with the cap open on a paper towel and tap to remove excess moisture.
 * 4) Add 250 uL of Buffer P1 to re-suspend bacteria in pellet. Mix by holding tubes by top and sliding across tube holder multiple times. Tap with finger to check that pellet is fully suspended.
 * 5) Add 250 uL of Buffer P2 and mix by inversion. The sample should turn viscous. Allow this to sit for approximately 3 minutes, but never exceed 5.
 * 6) Add 350 uL of Buffer N3 and mix by inversion to stop the lysis of cells. The solution should look chunky.
 * 7) Centrifuge for 10 minutes at 13,000 rpm.
 * 8) Add 800 uL of supernatant to a spin column. (Careful not to take the pellet!)
 * 9) Let stand for 30 seconds, then centrifuge for 30 seconds at 13,000 rpm.
 * 10) Take filtered liquid and place it in the filter again. Repeat the stand and spin process.
 * 11) Add any remaining supernatant to the filter and repeat this process of double filtering.
 * 12) Add 750 uL Buffer PE and let sit for 2 minutes.
 * 13) Centrifuge for 30 seconds at 13,000 rpm.
 * 14) Incubate with cap open at 65-70 degrees Celsius for 1 minute to dry the filter.
 * 15) Dry the membrane by centrifuging for 1 minute at top speed.
 * 16) Add 50 uL of elution buffer to filter and incubate with cap closed at 65-70 degrees Celsius for 4 minutes.
 * 17) Centrifuge at 0.5 x g for 1 minute, and 2 minutes at 13,000 rpm.
 * 18) Optional: place filtered solution back on filter and incubate again. Then centrifuge to elute.
 * 19) Measure the concentrations.