FM Preparation

FM Preparation – 4 Liters

1.     Obtain the 4 L container that is FM specific. This is located in the rear of the lab above the autoclave.

2.     Place the following things into the container

a. 2 packages of MS salts

b. 2 mL of Gamborgs Vitamin Solution 1000x

c. Large magnetic stir bar

3.     Dilute with 4 liters of Ultra-Pure H2O

4.     Stir with magnetic stirrer until all is dissolved.

5.     While stirring, use the FM specific pH electrode to bring the pH of the 4 liter solution to 6.5 using 1N KOH

6.     Pour the solution into the FM specific 1L black capped bottles.

7.     Autoclave on one cycle of 30 minutes @ 121°C.

8.     Test the FM with the Testing FM/CFM protocol.