Testing CFM and FM

Testing CFM and FM

1.     Obtain a vial of HNN wild-type spores.

2.     Obtain a 96 well plate.

3.     Make the four following solutions in a 50mL centrifuge tube:

a. 100 % CFM

b. 100 % FM

c. 50/50 – CFM/FM

d. 25/75 – CFM/FM

4.     Fill each well with 250 uL of each assigned solution. The well plate should be split up as shown by the diagram (click to zoom). 5.     Place a small amount of the spores onto a petri dish. Add the corresponding solution for which wells are being filled (ie. If the 100 % CFM wells are being filled, dilute with 100 % CFM).

6.     Using a P20, suck up a single spore and place it into a well.

7.     Repeat steps 5 and 6 as needed until each well is completely filled.