Spore sterilization


 * 1) Place spores in tube, size depending on the amount of spores.
 * 2) Add (at least 10x  spore volume) bleach solution, mix well and leave in bleach for 5 minutes.  Jody transfers the spore solution to new sterile tubes. Chao does it after step 3.
 * 3) Remove bleach from spores by placing sterile tip at bottom of tube and slowly sucking up the bleach.  The spores stay behind.
 * 4) Add sterile water, mix well, and remove water.  Repeat until spores have been washed three times.
 * 5) Add sterile water to spores.  The amount will depend on what you want to do with the spores.

Bleach solution:

10ml Chlorox concentrated bleach

20ml water

1 drop Tween 20