Fern media (FM)

0.5x MS salts plus vitamins (1x=4.13 g per liter). We purchase MS salts from PhytoTechnology (cat. M519)

pH 6.5 with KOH using the FM electrode.

It is important to use the FM electrode so that the FM does not get contaminated with antheridiogen!

For FM plates add 7g agar per liter FM.

It is important to use Sigma A1296 agar. We have tested other agars in the past and this one is the best because it's cheap and gametophytes grow well on it.