Preparing 1000x Ampicillin

Ampicillin Stock (1000x, makes 10 vials)

1.     Take Ampicillin powder from the -20°C and weigh out 1 g on the analytical balance.

2.     Place the weighed amount into a 15 mL conical vial that has 10 mL of water in it.

3.     Shake vigorously until all is dissolved.

4.     Obtain a 12 mL sterile syringe and .2 um sterile filter.

5.     Obtain a second 10 mL centrifuge tube.

6.     Clean and sterilize the hood.

7.     Open the syringe and remove the paper side of the filter package. Screw the two together.

8.     Remove the plunger from the syringe and pour the ampicillin/water mixture into the syringe.

9.     Place the second tube under the syringe and insert the plunger.

10.  Slowly press the plunger down until all the solution has flowed through.

11.  Using a P1000 pipette, place 1 mL of the solution into a 1.5 mL Eppendorf tube.

12.  Repeat until all of the filtered solution is gone.

This stock is 100mg/mL.

Add 1 eppendorf tube to 1 L of LB for the preparation of LB + Amp plates.