How to increase concentration of DNA miniprep

1. Make sure there is 3M NaOAc available

2. Add 1/10th volume of 3M NaOAc and mix well with pipetting

3. Add 2-2.5 volume of 100% EtOH and mix very well

4. Put the tubes in -80 freezer for at least 1hr, overnight is better for improving

4. Put the tubes and centrifuge at top speed for 20-30min (longer is better)

5. Remove supernatant without disturbing pellet

6. Add 100 μl of 70% cold EtOH

7. Centrifuge briefly (5-10 sec)

8. Remove supernatant